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  • Title: A simple and fast method for the simultaneous determination of polychlorinated biphenyls and polybrominated diphenyl ethers in small volumes of human serum.
    Author: Ramos JJ, Gómara B, Fernández MA, González MJ.
    Journal: J Chromatogr A; 2007 Jun 08; 1152(1-2):124-9. PubMed ID: 17204274.
    Abstract:
    A fast extraction and clean-up method for the simultaneous determination of PCBs and PBDEs has been developed. The procedure consisted of a solid-phase extraction (SPE) of the analytes on an Oasis HLB cartridge and the subsequent on-line fat elimination by directly dropping the eluate from the SPE cartridge onto a second cartridge containing layers of activated neutral silica gel and sulphuric acid modified silica gel. Detection limits using a gas chromatography coupled with an ion trap detector in the tandem mass spectrometry mode were from 0.03 to 0.3 pg/microL for PCBs and from 0.07 to 1.3 pg/microL for PBDEs. Repeatability (lower than 11%) and reproducibility (lower than 17%), expressed as relative standard deviation (RSD, n=4), were satisfactory. The feasibility of the method developed for the determination of the target compounds was evaluated by participation in several rounds of interlaboratory exercises involving human serum with a wide range of PBDE and PCB concentrations. The method has been applied to the evaluation of PBDEs and PCBs in human serum samples of up to 1 mL.
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