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  • Title: Radioprotectant screening for cryocrystallography.
    Author: Southworth-Davies RJ, Garman EF.
    Journal: J Synchrotron Radiat; 2007 Jan; 14(Pt 1):73-83. PubMed ID: 17211073.
    Abstract:
    Radiation damage continues to present a problem to crystallographers using cryocooled protein crystals at third-generation synchrotrons. Free-radical scavengers have been suggested as a possible means of reducing the rate of this damage. The screening of a large number of potential radioprotectants was undertaken with an online microspectrophotometer using cystine and cysteine to model protein disulfide bonds and thiol groups, respectively. Oxidized alpha-lipoic acid was tested as a possible model disulfide bond. The evidence for the effectiveness of ascorbate as a radioprotectant was strengthened, and quinone, 2,2,6,6-tetramethyl-4-piperidone, and reduced dithiothreitol showed promise as radioprotectants.
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