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  • Title: Enzyme-histochemical method for identification of lymphatic capillaries.
    Author: Kato S, Yasunaga A, Uchida U.
    Journal: Lymphology; 1991 Sep; 24(3):125-9. PubMed ID: 1721671.
    Abstract:
    Using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) double staining, lymphatic capillaries and blood capillaries were distinguished histochemically on cold glycol methacrylate (JB-4) sections of monkey intestines, on the basis of both enzyme characteristics. The specificity of the 5'-Nase reaction was obtained by inhibiting nonspecific ALPase in the 5'-Nase incubation medium including L-tetramisole. This double staining method demonstrated satisfactory isolated visualization of 5'-Nase activity in lymphatic capillaries and of ALPase activity in blood capillaries under light microscopy. The intensity and localization of 5'-Nase activity on the walls of lymphatic capillaries were also determined by the lead-based method under transmission electron microscopy. The intense reaction products of 5'-Nase activity were predominantly deposited on the outer surface of the plasma membrane of the lymphatic endothelial cells.
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