These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Random segment deletion based on IS31831 and Cre/loxP excision system in Corynebacterium glutamicum.
    Author: Tsuge Y, Suzuki N, Inui M, Yukawa H.
    Journal: Appl Microbiol Biotechnol; 2007 Apr; 74(6):1333-41. PubMed ID: 17221197.
    Abstract:
    A simple and random genome deletion method combining insertion sequence (IS) element IS31831 and the Cre/loxP excision system generated 42 Corynebacterium glutamicum mutants (0.2-186 kb). A total of 393.6 kb (11.9% of C. glutamicum R genome) coding for 331 genes was confirmed to be nonessential under standard laboratory conditions. The deletion strains, generated using only two vectors, varied not only in their lengths but also the location of the deletion along the C. glutamicum R genome. By comparing and analyzing the generated deletion strains, identification of nonessential genes, the roles of genes of hitherto unknown function, and gene-gene interactions can be easily and efficiently determined.
    [Abstract] [Full Text] [Related] [New Search]