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  • Title: Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass spectrometry.
    Author: Ding CG, Zhou Z, Ge QH, Zhi XJ, Ma LL.
    Journal: Biomed Chromatogr; 2007 Feb; 21(2):132-8. PubMed ID: 17221919.
    Abstract:
    A method has been developed for the simultaneous quantification of metformin (I) and glipizide (II) in human plasma. It is based on high-performance liquid chromatography with electrospray ionization tandem mass (LC-ESI-MS/MS) spectrometric detection in positive ionization mode. Phenformin (III) and gliclazide (IV) were used as internal standards for I and II, respectively. The MS/MS detection was performed in multiple reaction monitoring (MRM) mode. The precursor-product ion combinations of m/z 130 --> 71, 446 --> 321, 206 --> 60 and 324 --> 127 were used to quantify I, II, III and IV, respectively. This method was validated in the concentration ranges of 0.02-4 microg/mL for I and 0.004-0.8 microg/mL for II. It was utilized to support a clinical pharmacokinetic study after single dose oral administration of a combination of I and II.
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