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  • Title: Unexpected regioselectivity of Humicola insolens Cel7B glycosynthase mutants.
    Author: Blanchard S, Armand S, Couthino P, Patkar S, Vind J, Samain E, Driguez H, Cottaz S.
    Journal: Carbohydr Res; 2007 Apr 09; 342(5):710-6. PubMed ID: 17224137.
    Abstract:
    Four Humicola insolens Cel7B glycoside hydrolase mutants have been evaluated for the coupling of lactosyl fluoride on O-allyl N(I)-acetyl-2(II)-azido-beta-chitobioside. Double mutants Cel7B E197A H209A and Cel7B E197A H209G preferentially catalyze the formation of a beta-(1-->4) linkage between the two disaccharides, while single mutant Cel7B E197A and triple mutant Cel7B E197A H209A A211T produce predominantly the beta-(1-->3)-linked tetrasaccharide. This result constitutes the first report of the modulation of the regioselectivity through site-directed mutagenesis for an endoglycosynthase.
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