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  • Title: Bovine immunodeficiency virus: immunochemical characterization and serological survey.
    Author: Horzinek M, Keldermans L, Stuurman T, Black J, Herrewegh A, Sillekens P, Koolen M.
    Journal: J Gen Virol; 1991 Dec; 72 ( Pt 12)():2923-8. PubMed ID: 1722502.
    Abstract:
    Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with Mr values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population.
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