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  • Title: [Determination of plumbagin in different parts of Plumbago zeylanica by RP-HPLC].
    Author: Liu Y, Deng F, Liu C, Meng QY, Gao ZW.
    Journal: Zhongguo Zhong Yao Za Zhi; 2006 Oct; 31(20):1684-6. PubMed ID: 17225534.
    Abstract:
    OBJECTIVE: To develop a RP-HPLC method to determine plumbagin in Plumbago zeylanica, and to investigate contents of plumbagin in different parts of. P. zeylanica. METHOD: The analysis was carried out at 30 degrees C on a Kromasil C18, column eluted with a mobile phase consisting of a mixture of methanol-water (65: 35). The flow rate was 1 mL x min(-1), the detector wavelength was 213 nm. RESULT: The calibration curve was linear within the concentration ranges of 0.020 8-0. 104 microg (r = 0. 9999). The average recovery was 98.7%. The contents in the root, stem and leaf were 0.394 5%, 0.050 8%, 0.031 4% respectively. CONCLUSION: This method is simple, accurate, replicate and suitable for the determination of plumbagin in P. zeylanica.
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