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  • Title: [Determination of 3 kinds of components in huanglian jiedu decoction by HPLC under different UV wavelengths].
    Author: Li XZ, Lei P, Liu S.
    Journal: Zhongguo Zhong Yao Za Zhi; 2006 Oct; 31(20):1686-8. PubMed ID: 17225535.
    Abstract:
    OBJECTIVE: To determinate 3 kinds of effective components (5 compounds) in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baicalin and geniposide. METHOD: A HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, 238 nm for geniposide. Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, the mixture of H2O (A) , CH3OH (B) and 0.05% H3PO4 solution (C) as mobile phase in gradient mode. The column temperature was 35 degrees C and the flow rate was 1 mL x min(-1). RESULT: This method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately. CONCLUSION: The quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.
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