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  • Title: Functional identification of p-cumate operons in the terpene-degrading Rhodococcus sp. strain T104.
    Author: Bae HW, Kim D, Choi KY, Kwon NR, Chae JC, Zylstra GJ, Koh SC, Lee CH, Kim E.
    Journal: FEMS Microbiol Lett; 2007 Jan; 266(1):54-9. PubMed ID: 17233717.
    Abstract:
    We identified a p-cumate degrading gene cluster (designated cmt) with a novel organization in our genomic studies of the terpene-degrading Rhodococcus sp. strain T104. The mutant strain SN140, isolated for the inability to grow on p-cumate, accumulates 2,3-dihydroxy-p-cumate as identified by liquid chromatography-mass spectrometry and 300 MHz proton nuclear magnetic resonance spectroscopy. Reverse transcriptase polymerase chain reaction experiments showed that the cmt genes are operonic and induced specifically by growth on p-cumate. This report is the first example of identifying the genes for p-cumate degradation in a gram-positive organism based on functional data.
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