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  • Title: Lipolysis and proteolysis of modified and producer milks used for calibration of mid-infrared milk analyzers.
    Author: Kaylegian KE, Lynch JM, Fleming JR, Barbano DM.
    Journal: J Dairy Sci; 2007 Feb; 90(2):602-15. PubMed ID: 17235136.
    Abstract:
    Our objective was to determine if lipolysis or proteolysis of calibration sets during shelf life influenced the mid-infrared (MIR) readings or calibration slopes and intercepts. The lipolytic and proteolytic deterioration was measured for 3 modified milk and 3 producer milk calibration sets during storage at 4 degrees C. Modified and producer milk sets were used separately to calibrate an optical filter and virtual filter MIR analyzer. The uncorrected readings and slopes and intercepts of the calibration linear regressions for fat B, fat A, protein, and lactose were determined over 28 d for modified milks and 15 d for producer milks. It was expected that increases in free fatty acid content and decreases in the casein as a percentage of true protein of the calibration milks would have an effect on the MIR uncorrected readings, calibration slopes and intercepts, and MIR predicted readings. However, the influence of lipolysis and proteolysis on uncorrected readings was either not significant, or significant but very small. Likewise, the amount of variation accounted for by day of storage at 4 degrees C of a calibration set on the calibration slopes and intercepts was also very small. Most of the variation in uncorrected readings and calibration slopes and intercepts were due to differences between the optical filter and virtual filter analyzers and differences between the pasteurized modified milk and raw producer milk calibration sets, not due to lipolysis or proteolysis. The combined impact of lipolysis and proteolysis on MIR predicted values was <0.01% in most cases.
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