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  • Title: Supramolecular-mediated immobilization of L-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications.
    Author: Villalonga R, Fujii A, Shinohara H, Asano Y, Cao R, Tachibana S, Ortiz P.
    Journal: Biotechnol Lett; 2007 Mar; 29(3):447-52. PubMed ID: 17237972.
    Abstract:
    A supramolecular approach was used for adsorbing a monolayer of adamantane-modified phenylalanine dehydrogenase on beta-cyclodextrin-coated Au electrodes. The enzyme electrode (poised at +200 mV vs. Ag/AgCl) showed a linear amperometric response up to 3 mM L-phenylalanine (L-Phe) with a lower detection limit of 15 microM. The reversible nature of this immobilization approach was confirmed.
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