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  • Title: Elevation of adenosine 3',5'-cyclic monophosphate in established mamalian cell strains by Hypaque (sodium diatrizoate).
    Author: Patrick JC, Rengachary S, Melnykovych G.
    Journal: In Vitro; 1975; 11(6):404-8. PubMed ID: 172435.
    Abstract:
    Hypaque (sodium diatrizoate), when added to cells growing in culture (two HeLa S3 strains, rat glioblastoma C6 and mouse lymphoma L5178Y), increased the intracellular level of adenosine 3',5'-cyclic monophosphate. A transient elevation of adenosine 3',5'-cyclic monophosphate was also observed when L5178Y cells were subjected to a procedure recommended for separation of lymphocytes from peripheral blood. The effect of Hypaque did not appear to be related to the increase in osmolality of the medium.
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