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  • Title: Biosynthesis of intracellular 5-aminolevulinic acid by a newly identified halotolerant Rhodobacter sphaeroides.
    Author: Tangprasittipap A, Prasertsan P, Choorit W, Sasaki K.
    Journal: Biotechnol Lett; 2007 May; 29(5):773-8. PubMed ID: 17245554.
    Abstract:
    Of 23 strains of halotolerant (up to 12% w/v NaCl) photosynthetic bacteria isolated from various sources, one isolate, SH5, accumulated intracellular 5-aminolevulinic acid (ALA) at 0.45 microg/g dry cell wt (DCW) growing aerobically in the dark. The strain was identified as Rhodobacter sphaeroides using 16S rDNA sequencing. Biosynthesis of ALA was enhanced to 14 microg/g DCW using modified glutamate/glucose (50 mM) medium with the addition of 10 mM levulinic acid after 24 h cultivation. Addition of 30 microM Fe(2+) to this medium increased the yield to 226 microg/g DCW.
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