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  • Title: [The structure and composition of the complex that provides the adhesion of the cellular layers during the morphogenesis of the human embryonic adenohypophysis].
    Author: Bozhok IuM, Kozyritskiĭ VG, Zelinskaia AV.
    Journal: Ontogenez; 1991; 22(6):565-74. PubMed ID: 1724695.
    Abstract:
    The area of contact between adenohypophysis and diencephalon rudiments of human embryos (5-7 weeks of development) was studied using immunohistochemistry and electron microscopy. Basement membranes of Rathke's pouch ectoderm and of diencephalon bottom neuroectoderm are connected by means of a complex consisting of thin fibrous material and collagen-like fibrils. After Ca+2 and Mg+2 ions removal, this complex with basement membranes was separated from epithelial layers. The material in the contact area differed in its composition from the basement membranes covering the adenohypophysis and diencephalon rudiments by the high content of tenascin and the absence of EDB-fibronectin. Other basement membrane components (collagen IV, heparan-sulphate proteoglycans, entactin and laminin) were also present in this area. Tenascin accumulation was also found in Rathke's pouch epithelium and cavity.
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