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  • Title: Assembly of carboxy-terminally deleted desmin in vimentin-free cells.
    Author: Raats JM, Henderik JB, Verdijk M, van Oort FL, Gerards WL, Ramaekers FC, Bloemendal H.
    Journal: Eur J Cell Biol; 1991 Oct; 56(1):84-103. PubMed ID: 1724756.
    Abstract:
    Using a vimentin-free expression system we were able to demonstrate that the carboxy terminus of desmin is necessary for filament assembly in the living cell. Desmin subunits missing only 4 carboxy-terminal residues of their rod domain are incapable of homopolymeric filament assembly. Moreover, even single amino acid substitutions in the conserved carboxy-terminal part of the rod domain prevent desmin subunits from homopolymeric filament assembly. Desmin subunits missing 18 or more carboxy-terminal residues of their rod domain (including the complete conserved carboxy-terminal region) are unstable in cells devoid of intact type III intermediate filaments (IFs). Interaction with an intact type III IF, however, stabilizes these mutated desmin subunits. Expression of a desmin subunit missing both its non-helical end domains in vimentin-containing cells disrupts the endogenous vimentin network completely.
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