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  • Title: Characterization of the SOS response of Pseudomonas fluorescens strain DC206 using whole-genome transcript analysis.
    Author: Jin H, Retallack DM, Stelman SJ, Hershberger CD, Ramseier T.
    Journal: FEMS Microbiol Lett; 2007 Apr; 269(2):256-64. PubMed ID: 17250760.
    Abstract:
    DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the LexA repressor protein. Interestingly, expression of 33 clustered bacteriophage-like genes was upregulated, suggesting that mitomycin C (MMC) may induce a prophage resident in the P. fluorescens genome. However, no phage particles were detected in P. fluorescens strain DC206 that had been treated with MMC using transmission electron microscopy. The same preparation failed to produce phage plaques on lawns of any of 10 different Pseudomonas strains tested, indicating that the 33 bacteriophage-like gene cluster represents a defective prophage.
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