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  • Title: [The detection of the Marburg virus antigen by solid-phase immunoenzyme analysis].
    Author: Vladyko AS, Chepurnov AA, Bystrova SI, Lemeshko NN, Lukashevich IS.
    Journal: Vopr Virusol; 1991; 36(5):419-21. PubMed ID: 1725078.
    Abstract:
    Comparative studies of two variants of the enzyme-linked immunosorbent assay (ELISA) were carried out to determine the sensitivity of the detection of Marburg virus antigens in Vero cells. Both competitive and two-antibody ELISA variants detected as little as 5 ng of Marburg virus antigen. The Vero cell monolayer was found to produce 5-50 ng/0.05 ml of the virus-specific proteins at 6 to 8 days postinfection.
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