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  • Title: Miniaturised isotachophoresis of DNA.
    Author: Prest JE, Baldock SJ, Day PJ, Fielden PR, Goddard NJ, Treves Brown BJ.
    Journal: J Chromatogr A; 2007 Jul 13; 1156(1-2):154-9. PubMed ID: 17254590.
    Abstract:
    This paper presents the findings of a feasibility study investigating the behaviour of DNA under conditions of miniaturised isotachophoresis. An electrolyte system comprising a leading electrolyte of 5mM perchloric acid at pH 6.0 and a terminating electrolyte of 10mM gallic acid was devised and used to perform isotachophoresis of DNA containing samples on a miniaturised poly(methyl methacrylate) device. Under such conditions it was found that no separation of DNA fragments was observed with the substance migrating instead as a single isotachophoretic zone. Whilst such a result shows the method is unsuitable for analysis DNA it offers significant potential as a means of sample preparation for subsequent analysis using another method. This is because the single zone of DNA formed is preconcentrated to a constant concentration governed by the leading ion and is separated from all species with different effective electrophoretic mobilities.
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