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  • Title: Production and characterization of mouse monoclonal antibodies against the transmembrane protein of a human immunodeficiency virus type 2.
    Author: Komatsu H, Yamashita A, Tozawa H, Mizutani Y, Honda M, Kawamura M, Hayami M.
    Journal: AIDS Res Hum Retroviruses; 1991 Dec; 7(12):999-1005. PubMed ID: 1725959.
    Abstract:
    We established seven hybridoma clones producing monoclonal antibodies (MAbs) against the envelope transmembrane protein (TMP) of a Ghanian isolate of human immunodeficiency virus type 2 (HIV-2[GH-1]) from mice immunized with the detergent-disrupted purified whole virus. The MAbs were found to react with 35 kilodalton (kD) TMP of the HIV-2[GH-1] virus in a Western blot assay (WB). Two of these MAbs recognized 135 kD proteins in addition to TMP in the lysate of HIV-2[GH-1]-infected cells. Two other MAbs cross-reacted with viral components corresponding to TMPs of HIV-2ROD and SIVMAC isolates in a Western blot. Results of competitive binding assay suggest that there are at least three epitopes on a TMP molecule of the HIV-2[GH-1] isolate. The MAbs did not inhibit syncytium formation between HIV-2[GH-1]-infected MOLT-4 cells and MOLT-4 clone 8 cells, nor virus infection to MOLT-4 clone 8 cells.
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