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  • Title: [Detection of cytomegalovirus in urine by the shell-vial technique: usefulness of diluting the sample].
    Author: Acosta B, Ferrer D, Jordán M, González D, Gobernado M.
    Journal: Enferm Infecc Microbiol Clin; 1991 Nov; 9(9):561-3. PubMed ID: 1726602.
    Abstract:
    To assess the toxicity of urine samples on human fibroblast cell monolayer using Shell-vial technique for cytomegalovirus antigen detection. A total of 227 urine samples were processed, inoculated on Shell-vial simultaneously, being either diluted (1:1) and non diluted in transport media. After 24-48 hours incubation, the fibroblast cells monolayer was stained using fluorescein-marked monoclonal anti-cytomegalovirus antibodies. Cytomegalovirus was detected in 9.6% of samples inoculated with diluted urine, and in 5.2% of samples inoculated with non-diluted urine. Destruction of monolayer cells was seen in 13.2 and 22.4, respectively. With Shell-vial technique for detecting cytomegalovirus in urine samples, a high number of positive results was obtained using diluted urine. Also a significant minor destruction of cells monolayer was observed with the same inoculum.
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