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  • Title: Accurate assessment of cell count and viability with a flow cytometer.
    Author: Shenkin M, Babu R, Maiese R.
    Journal: Cytometry B Clin Cytom; 2007 Sep; 72(5):427-32. PubMed ID: 17266154.
    Abstract:
    BACKGROUND: In this study we developed a method to measure cell concentration and viability in specimens received in flow cytometry and cytogenetics laboratories. METHODS: Specimens are stained with a vital fluorescent dye, SYTO13, the cell impermeant viability dye, 7-AAD, and a leukocyte marker, CD45. After the addition of an internal calibrator microsphere, FLOW-COUNT, the flow cytometer is capable of measuring the viability of nucleated cells, giving a general assessment of leukocyte populations and measuring their concentration. RESULTS: An accurate assessment of specimen quality is an important parameter when performing flow cytometric and cytogenetic leukemia/lymphoma assessment. High quality specimen is desired to avoid the pitfalls of non-specific staining and limited cellularity/viability. CONCLUSIONS: Use of a cell count and viability measurement prior to leukemia and lymphoma assessment by flow cytometry and cytogenetics helps to increase the rate of successful immunophenotypic and cytogenetic analysis.
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