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  • Title: Electrochemical detection in a microfluidic device of oxidative stress generated by macrophage cells.
    Author: Amatore C, Arbault S, Chen Y, Crozatier C, Tapsoba I.
    Journal: Lab Chip; 2007 Feb; 7(2):233-8. PubMed ID: 17268626.
    Abstract:
    The release of reactive oxygen species (ROS) or reactive nitrogen species (RNS), i.e., the initial phase of oxidative stress, by macrophage cells has been studied by electrochemistry within a microfluidic device. Macrophages were first cultured into a detection chamber containing the three electrodes system and were subsequently stimulated by the microinjection of a calcium ionophore (A23187). Their production of ROS and RNS was then measured by amperometry at the surface of a platinized microelectrode. The fabricated microfluidic device provides an accurate measurement of oxidative release kinetics with an excellent reproducibility. We believe that such a method is simple and versatile for a number of advanced applications based on the detection of biological processes of secretion by a few or even a single living cell.
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