These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Trends in sample preparation for classical and second generation proteomics.
    Author: Cañas B, Piñeiro C, Calvo E, López-Ferrer D, Gallardo JM.
    Journal: J Chromatogr A; 2007 Jun 15; 1153(1-2):235-58. PubMed ID: 17276441.
    Abstract:
    Sample preparation is a fundamental step in the proteomics workflow. However, it is not easy to find compiled information updating this subject. In this paper, the strategies and protocols for protein extraction and identification, following either classical or second generation proteomics methodologies, are reviewed. Procedures for: tissue disruption, cell lysis, sample pre-fractionation, protein separation by 2-DE, protein digestion, mass spectrometry analysis, multidimensional peptide separations and quantification of protein expression level are described.
    [Abstract] [Full Text] [Related] [New Search]