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  • Title: Purification of vaccinia virus by zonal centrifugation and analysis of viral protein composition.
    Author: Madaliński W, Bańkovski A, Korbecki M.
    Journal: Acta Virol; 1977 Mar; 21(2):104-8. PubMed ID: 17277.
    Abstract:
    Vaccinia virus propagated in rotated cultures of RK13 cells was purified by sucrose density gradient zonal centrifugation. The purified virus was dissociated with sodium dodecyl sulfate end its protein composition analyzed by polyacrylamide gel electrophoresis. The protein composition of the virion envelope, isolated by the non-ionic detergent Nonidet P-40, was also determined.
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