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  • Title: Monitoring the reaction of hemoglobin with hydrogen peroxide by capillary electrophoresis-chemiluminescence detection.
    Author: Zhou SL, Wang JH, Huang WH, Lu X, Cheng JK.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2007 May 01; 850(1-2):343-7. PubMed ID: 17280882.
    Abstract:
    The reaction of hemoglobin (Hb) with hydrogen peroxide (H2O2) leads to fluorescent product and heme degradation. We applied capillary electrophoresis-chemiluminescence (CE-CL) detection to monitor the course of Hb reacting with H2O2. Hb and released free iron ion (Fe3+) were detected based on their enhancement effects on CL of the luminol-H2O2 system. In this study, we discovered an intermediate of this reaction which intensely enhances the luminol-H2O2 CL system. The ratio of max CL signals of Fe3+, Hb and this intermediate is circa 1:10:60.
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