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  • Title: An artificial liver sinusoid with a microfluidic endothelial-like barrier for primary hepatocyte culture.
    Author: Lee PJ, Hung PJ, Lee LP.
    Journal: Biotechnol Bioeng; 2007 Aug 01; 97(5):1340-6. PubMed ID: 17286266.
    Abstract:
    Primary hepatocytes represent a physiologically relevant model for drug toxicity screening. Here, we created a biologically inspired artificial liver sinusoid with a microfluidic endothelial-like barrier having mass transport properties similar to the liver acinus. This unit consisted of a cord of hepatocytes (50 x 30 x 500 microm) fed by diffusion of nutrients across the microfluidic endothelial-like barrier from a convective transport vessel (10 nL/min). This configuration sustained rat and human hepatocytes for 7 days without an extracellular matrix (ECM) coating. Experiments with the metabolism mediated liver toxicant diclofenac showed no hepatotoxicity after 4 h and an IC(50) of 334 +/- 41 microM after 24 h.
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