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  • Title: Quantitative surface-enhanced resonance Raman scattering of phthalocyanine-labelled oligonucleotides.
    Author: Macaskill A, Chernonosov AA, Koval VV, Lukyanets EA, Fedorova OS, Smith WE, Faulds K, Graham D.
    Journal: Nucleic Acids Res; 2007; 35(6):e42. PubMed ID: 17289751.
    Abstract:
    The evaluation of phthalocyanine labels for the surface-enhanced resonance Raman scattering (SERRS) detection of oligonucleotides is reported. Three phthalocyanine-labelled oligonucleotides were assessed, each containing a different metal centre. Detection limits for each labelled oligonucleotide were determined using two excitation frequencies where possible. Limits of detection as low as 2.8 x 10(-11) mol. dm(-3) were obtained which are comparable to standard fluorescently labelled probes used in previous SERRS studies. The identification of two phthalocyanine-labelled oligonucleotides without separation was also demonstrated indicating their suitability for multiplexing. This study extends the range of labels suitable for quantitative surface-enhanced resonance Raman scattering with silver nanoparticles and offers more flexibility and choice when considering SERRS for quantitative DNA detection.
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