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  • Title: A self-immolative reporter for beta-galactosidase sensing.
    Author: Ho NH, Weissleder R, Tung CH.
    Journal: Chembiochem; 2007 Mar 26; 8(5):560-6. PubMed ID: 17300128.
    Abstract:
    A dual fluorogenic and chromogenic probe, Gal-2SBPO, has been developed based on a synthesized water-soluble dye, 9-di-3-sulfonyl-propylaminobenzo[a]phenoxazonium perchloride (2SPBO, lambda(ex/em)=630/670 nm). beta-D-Galactopyranoside, the substrate of beta-galactosidase, was conjugated to 2SBPO through a para-substituted benzyloxycarbonyl group and a glycine residue, which serve as a self-immolative spacer and as a molecular blocker to mask the optical signal of 2SBPO, respectively. Gal-2SBPO was soluble and stable under physiological conditions. Enzymatic cleavage of the beta-D-galactopyranoside triggered a series of spontaneous reactions that resulted in a release of optically active 2SBPO. The beta-galactosidase activity was assayed by monitoring the absorbance at 630 nm and fluorescence at 670 nm.
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