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  • Title: [Analysis of coumarins in Fructus Cnidii by HPLC-ESI-MS].
    Author: Jiang YQ.
    Journal: Zhong Yao Cai; 2006 Oct; 29(10):1033-5. PubMed ID: 17326403.
    Abstract:
    OBJECTIVE: To analyze coumarins in Fructus Cnidii by HPLC-ESI-MS. METHODS: Coumarins in Fructus Cnidii were subjected to HPLC, using Shim-pack CLC-ODS column, methanol-water (70:30) as mobile phase, and 254 nm as DAD wavelength. A quadrupole mass spectrometer was applied to verify the LC elute, operated in the positive ion mode. RESULTS: Three coumarins were determined and the possible structures of seven coumarins were deduced based on the chromatography and MS data. CONCLUSION: The application of this method could be available for the rapid identification of constituents from traditional Chinese medicines.
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