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  • Title: [Isolation and characterization of human colorectal cancer cell subline with unique metastatic potential in the liver].
    Author: Zhang YF, Liu L, Ding YQ.
    Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2007 Feb; 27(2):126-30. PubMed ID: 17355917.
    Abstract:
    OBJECTIVE: To isolate a subclone from human colorectal cancer cell line SW480 with unique metastatic potential in the liver. METHODS: Human colorectal cancer cells SW480 were implanted into BALB/c nude mice, and the hepatic metastatic lesions were harvested, and the tumor tissue blocks were re-implantated into nude mice for a second round of in vivo selection. The same procedure was repeated 5 times until a new cell subline, designated as SW480/M5, was established, whose biological behaviors was investigated both in vivo and in vitro. RESULTS: After orthotopic implantation of the tumor tissue into the colon of nude mouse for 28 days, widespread loco-regional and distant metastases occurred in mice inoculated with SW480 tissue, and those inoculated with SW480/M5 tissue had hepatic metastasis only. Compared with the parental cells, SW480/M5 cells were characterized by greater clonality, reproductive activity, motor ability, invasiveness, and weaker adhesive capacity to fibronectin. CONCLUSION: A human colorectal cancer cell subline with unique liver metastatic potential has been established by in vivo selection to serve as a new model for study ing colorectal cancer metastasis.
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