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  • Title: Determination of paroxetine in human saliva by reversed-phase high-performance liquid chromatography with UV detection.
    Author: Tsuruta T, Yang C, Ueki H, Li G, Maekawa A, Kamikawa H, Oku E, Somehara T, Fujito H, Tatebayashi H, Yamada S.
    Journal: Nihon Shinkei Seishin Yakurigaku Zasshi; 2007 Feb; 27(1):9-12. PubMed ID: 17393774.
    Abstract:
    A rapid and sensitive high-performance liquid chromatographic method was validated and described for determination of paroxetine in human saliva. Following liquid-liquid extraction of the drug and an internal standard (dibucaine), chromatographic separation was accomplished using a C18 analytical column with a mobile phase consisting of 0.05 mol/L sodium phosphate buffer, pH 5.0, and acetonitrile (A 30:70, v/v; B 60:40, v/v). Paroxetine and the internal standard were detected by ultraviolet absorbance at 205 nm. The average recoveries of the drug and internal standard were 92.5% and 89%, respectively. The lower limits of detection and quantification were 1 and 4 ng/ml, respectively, and the calibration curve was linear over a concentration range of 4 ng/ml. The saliva level of paroxetine in patients with depression taking 10 to 40 mg/day of the drug was significantly correlated with the plasma level of paroxetine in each patient (r = 0.617, P < 0.004, n = 19). These data indicate that the saliva level of paroxetine could be a useful marker to predict the plasma level of the drug.
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