These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Time-related changes in functional activity and capacitation of chinchilla (Chinchilla lanigera) spermatozoa during in vitro incubation.
    Author: Ponzio MF, Busso JM, Ruiz RD, de Cuneo MF.
    Journal: Anim Reprod Sci; 2007 Dec; 102(3-4):343-9. PubMed ID: 17395408.
    Abstract:
    The application of assisted breeding programs for chinchilla, an endangered species, requires detailed knowledge about their gamete physiology. Main purposes of the present study were to examine the time-related changes during 8h in vitro incubation in parameters that reflect chinchilla sperm functional activity (including sperm motility, viability, membrane and acrosome integrity), and to determine the incubation time required for achieving in vitro sperm capacitation, evaluated through the quantification of the percentages of sperm that underwent the acrosome reaction in response to progesterone (P, 20 microM) or another acrosome reaction inducer the calcium ionophore, A23187 (20 nM). Semen was obtained by electroejaculation, subjected to swim-up and incubated for 0, 2, 4 and 8h. After these periods, sperm functional activity was assessed. In all treatments percentages of motile, viable and viable sperm with intact acrosomes decreased (p<0.001) after 8h of incubation. The percentages of swollen gametes decreased (p<0.001) after 2h of incubation. Capacitation of chinchilla sperm could be achieved within 4h, as indirectly demonstrated by the increase of acrosome reacted cells in response to P or A23187 (time x treatment interaction: p=0.02).
    [Abstract] [Full Text] [Related] [New Search]