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  • Title: Matrix metalloproteinases and inhibitor in knee synovial fluid as cartilage biomarkers in rabbits: the effect of high-intensity jumping exercise.
    Author: Qi C, Changlin H, Zefeng H.
    Journal: J Surg Res; 2007 Jun 01; 140(1):149-57. PubMed ID: 17397871.
    Abstract:
    OBJECTIVE: To study the cartilage injury of knee joint in a rabbit model under high-intensity jumping training and to investigate if levels of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in synovial fluid (SF) can be used to predict early sports injury of articular cartilage effectively. MATERIALS AND METHODS: Forty New Zealand white rabbits were divided into two groups randomly: untreated control group (CTRL, n = 8) and jumping training group (TG, n = 32). Concentrations of MMP-1, MMP-3, and TIMP-1 in SF were measured by ELISA assays at 4 and 8 weeks, respectively. Rabbits were euthanized at 4 and 8 weeks, and knee joints were taken out to be examined histologically. Sulfated glycosaminoglycan (GAG) content, thickness of cartilage and subchondral bone, dead cell ratio, and Mankin grades were measured. RESULTS: At 4 weeks, sulfated GAG content, thickness of subchondral bone, and Mankin grades in TG were significantly higher than control. After 8 weeks, the TG cohort had a further increase in the articular cartilage injury. SF levels of MMP-3, TIMP-1, and MMP-3/TIMP-1 in TG were significantly higher than control, and the level of these biomarkers was significantly associated with the severity of the articular cartilage pathology. CONCLUSIONS: Repetitive and high-intensity jumping movement may induce sports injury in the knee joint cartilage. MMP-1, MMP-3, TIMP-1, and MMP-3/TIMP-1 in the SF may accurately predict the severity and pathological characteristic of the joint cartilage injury.
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