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  • Title: Crystallization of porcine pancreatic elastase and a preliminary neutron diffraction experiment.
    Author: Kinoshita T, Tamada T, Imai K, Kurihara K, Ohhara T, Tada T, Kuroki R.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2007 Apr 01; 63(Pt 4):315-7. PubMed ID: 17401204.
    Abstract:
    Porcine pancreatic elastase (PPE) resembles the attractive drug target leukocyte elastase, which has been implicated in a number of inflammatory disorders. In order to investigate the structural characteristics of a covalent inhibitor bound to PPE, including H atoms and the hydration by water, a single crystal of PPE for neutron diffraction study was grown in D(2)O containing 0.2 M sodium sulfate (pD 5.0) using the sitting-drop vapour-diffusion method. The crystal was grown to a size of 1.6 mm(3) by repeated macroseeding. Neutron diffraction data were collected at room temperature using a BIX-3 diffractometer at the JRR-3 research reactor of the Japan Atomic Energy Agency (JAEA). The data set was integrated and scaled to 2.3 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 51.2, b = 57.8, c = 75.6 A.
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