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  • Title: Synthesis of TOAC spin-labeled proteins and reconstitution in lipid membranes.
    Author: Karim CB, Zhang Z, Thomas DD.
    Journal: Nat Protoc; 2007; 2(1):42-9. PubMed ID: 17401337.
    Abstract:
    A procedure is described for the synthetic incorporation into membrane proteins of the non-natural amino acid TOAC (2,2,6,6-tetramethyl-piperidine-1-oxyl-4-amino-4-carboxylic acid), which is coupled rigidly to the alpha-carbon, providing direct detection of peptide backbone dynamics by electron paramagnetic resonance (EPR). Also included is a protocol for the functional reconstitution of the spin-labeled protein in lipid vesicles. This protocol can be completed in 17 d.
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