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  • Title: [Erythropoietin prevents oxidative stress and lipid peroxidation process in human umbilical vein endothelial cells induced by tumor necrosis factor alpha].
    Author: Szczepański M, Kamianowska M, Wnuczko K, Skrzydlewska E, Wołczyński S.
    Journal: Pol Merkur Lekarski; 2006 Dec; 21(126):534-9. PubMed ID: 17405292.
    Abstract:
    UNLABELLED: According last research erythropoietin (Epo), glycoprotein known as a physiological regulator of erythropoiesis and used successfully to treat anemia, displays cytoprotective properties. It is supposed that the protective effect of EPO is based on its ability to oxidation-reduction stabilization of cells. AIM: The aim of the study was to examine the influence of oxidative stress and lipid peroxidation in human umbilical vein endothelial cells (HUVEC) induced by tumor necrosis factor-alpha (TNF-alpha). MATERIAL AND METHODS: Human umbilical vein endothelial cells were cultured in a standard medium (M 199). The experiment was performed five times on the forth passage of HUVEC culture. For stimulation TNF-alpha was used in concentrations: 10, 20, 40 ng/ml (6 hours) and erythropoietin in concentrations: 10, 20, 40 IU/ml (24 hours). In the HUVEC lysate malonyldialdehyde (MDA), lipid hydroperoxides and reduction gluthation (GSH) concentrations were measured by HPLC method. RESULTS: MDA concentration statistically significantly decreased from 16.48 +/- 1.21 to 14.40 +/- 0.72 nmol/mg protein and LOOH concentration from 73.00 +/- 5.44 do 68.86 +/- 1.89 nmol/mg protein in the cell's lysate due to the preincubation with Epo of HUVEC cells, stimulated with TNF-alpha. MDA and LOOH concentrations in the control culture were 12.91 +/- 1.02 nmol/mg protein and 57.80 +/- 6.16 nmol/mg protein respectively. The application of erythropoietin while the cells were being stimulated with TNF-alpha prevented the decrease in GSH concentration which was 34.77 +/- 0.70 nmol/mg protein in the control culture, 33.11 +/- 1.65 nmol/mg protein in the culture stimulated with TNF-alpha and 34.17 +/- 0.14 nmol/mg protein in culture preincubated with Epo and stimulated by TNF-alpha. CONCLUSIONS: Erythropoietin (Epo) prevents oxidative stress and lipid peroxidation process in human umbilical vein endothelial cells (HUVEC) induced by tumor necrosis factor-alpha.
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