These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: An anion-exchange high-performance liquid chromatography method coupled to total organic carbon determination for the analysis of water-soluble organic aerosols.
    Author: Mancinelli V, Rinaldi M, Finessi E, Emblico L, Mircea M, Fuzzi S, Facchini MC, Decesari S.
    Journal: J Chromatogr A; 2007 May 18; 1149(2):385-9. PubMed ID: 17412354.
    Abstract:
    The chemical composition of water-soluble organic carbon (WSOC) in atmospheric aerosol particles is largely unexplored, due to the myriad of individual compounds, which has hampered attempts to attain a full characterization at the molecular level. An alternative approach, focusing on the analysis of a few main chemical classes, allowed the quantitative fractionation of WSOC into neutral compounds (NC), mono- and di-acids (MDA) and polyacids (PA) through an anion-exchange liquid chromatographic method. Previous attempts to quantify NC, MDA and PA relied on a low-pressure chromatographic technique using a volatile buffer, followed by total organic carbon (TOC) analysis of the fractions, or alternatively on a faster HPLC-UV method which provided a quantification of the fractions based on empirical relationships between UV signal and TOC concentration. Here, we report an upgraded anion-exchange HPLC technique, allowing direct TOC analysis of the eluted fractions, without any pre-treatment, thus permitting a great simplification of quantitative analysis and preventing sample losses. The new HPLC-TOC methodology, employing completely inorganic eluents shows the same efficiency of the former HPLC-UV method employing organic additives, with the exception of phenolic compounds, which are retained on the column by secondary interactions, and low-molecular weight tricarboxylic acids, which are recovered among MDA. The new anion-exchange liquid chromatographic method can recover a substantial amount (86-100%) of water-soluble organic carbon from atmospheric aerosol extracts, thus allowing a higher retention and fractionation capacity with respect to alternative techniques, like solid phase extraction.
    [Abstract] [Full Text] [Related] [New Search]