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  • Title: [Experssion of human glutathione-S-transferase A1 in CHO cells].
    Author: Chen P, Wang Q, Li W, Cui L.
    Journal: Wei Sheng Yan Jiu; 2007 Jan; 36(1):16-9. PubMed ID: 17424839.
    Abstract:
    OBJECTIVE: To establish the Chinese Hamster Ovary (CHO) expression system for the human glutathione-S-transferase (GST) A1. METHODS: CHO cells was transfected with recombinant vector pDNA3.1- GSTA1 by lipofectamineTM 2000. The expression of selected CHO-GSTA1 in G418-resistant clones were determined by PCR, RT-PCR and Western blot. CHO-GSTA1 high-expressed clone was cultured to collect supernatant and activity was assayed by U-2001 ultraviolet spectrophotometer. RESULTS: CHO-GSTA1 was obtained by PCR, RT-PCR and Western blot. The expressed protein of GSTA1 had biological activity, it was 35 mmol/(mg min pro). CONCLUSION: The CHO-GSTA1 was constructed and for research purification protein and candidate strains of detoxicitic mechanism of chemical toxins and carcinogenesis.
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