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  • Title: [Expression, purification, and characterization of an anti-human RBC ScFv-HIV gp160 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood].
    Author: Hu Y, Yang JY, Zhu L, Hou J, Shen HH, Mao PY.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2007 Mar; 21(1):76-8. PubMed ID: 17429544.
    Abstract:
    OBJECTIVE: To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV. METHODS: The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E. coli. RESULTS: The fusion protein was proved to be able to bind both anti-RBC and HIVgp160. It could cause agglutination of human RBC when HIVgp160 was present. CONCLUSION: The fusion protein has the potential in rapid detection of HIV.
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