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  • Title: Evaluation of immunoassay-based MMP-8 detection in gingival crevicular fluid on a point-of-care platform.
    Author: Munjal SK, Prescher N, Struck F, Sorsa T, Maier K, Netuschil L.
    Journal: Ann N Y Acad Sci; 2007 Mar; 1098():490-2. PubMed ID: 17435156.
    Abstract:
    A novel immunology-based point-of-care test has been designed to assess the activated form of matrix metalloproteinase-8 (aMMP-8) for diagnosis and monitoring of periodontal diseases. The test has been automated using an analyzer, which quantitatively measures aMMP-8 in 18 min in gingival crevicular fluid (GCF). Fluid samples were collected from healthy, gingivitis-, and periodontitis-affected teeth. The test results from the analyzer were compared with quantitative aMMP-8 immunofluorometric assay (IFMA) and in-house enzyme-linked immunosorbent assay (ELISA) as well as with the periodontal state. Preliminary results of analyzer measurements of these 34 clinical samples showed a good agreement with the results from IFMA and in-house ELISA and with the clinical picture.
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