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  • Title: Multiplexed single-molecule assay for enzymatic activity on flow-stretched DNA.
    Author: Kim S, Blainey PC, Schroeder CM, Xie XS.
    Journal: Nat Methods; 2007 May; 4(5):397-9. PubMed ID: 17435763.
    Abstract:
    We report a single-molecule assay for nucleic-acid enzymes on flow-stretched DNA templates. To facilitate the detection of slow or intermittent enzymatic activities, we developed the assay with 15-nm spatial resolution at a frame rate of 1 Hz and approximately 10 nm mechanical stability over the timescale of hours. With multiplexed data collection, we applied the assay to phi29 DNA polymerase, HIV-1 reverse transcriptase, lambda exonuclease and Escherichia coli RNA polymerase.
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