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  • Title: Fast determination of conditions for maximum dynamic capacity in cation-exchange chromatography of human monoclonal antibodies.
    Author: Faude A, Zacher D, Müller E, Böttinger H.
    Journal: J Chromatogr A; 2007 Aug 17; 1161(1-2):29-35. PubMed ID: 17442329.
    Abstract:
    Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to the Hofmeister series. As this downshift correlates well with zeta potential values, a measurement of the latter allows the determination of the pH value for maximum DBC under a given set of conditions. Thus, the use of zeta potential values can accelerate the purification process development and helps to understand the protein adsorption mechanism.
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