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  • Title: Fourier transform infrared imaging as a method for detection of HLA class I expression in melanoma without the use of antibody.
    Author: Chew SF, Wood BR, Kanaan C, Browning J, MacGregor D, Davis ID, Cebon J, Tait BD, McNaughton D.
    Journal: Tissue Antigens; 2007 Apr; 69 Suppl 1():252-8. PubMed ID: 17445214.
    Abstract:
    Human leukocyte antigen (HLA) class I expression in melanoma is usually assessed using immunohistochemical staining. Here we report on the use of Fourier transform infrared (FTIR) hyperspectral imaging, a method widely used in two-dimensional analysis of chemical components, to study HLA class I expression in tissue. Two-dimensional cluster colour images derived from unsupervised hierarchical cluster analysis of FTIR hyperspectral data on melanoma sections were compared with consecutive sections that were immunohistochemically stained for class I expression. HLA-class-I-positive and -negative areas were differentiated by FTIR cluster images in all eight melanoma sections investigated without the need for antibody attachment. FTIR imaging enables the distinction of HLA-class-I-positive from class-I-negative areas in melanoma. This method is accurate, rapid and cost-effective.
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