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  • Title: Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent.
    Author: Ainsworth EA, Gillespie KM.
    Journal: Nat Protoc; 2007; 2(4):875-7. PubMed ID: 17446889.
    Abstract:
    Non-structural phenolic compounds perform a variety of functions in plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay that utilizes Folin-Ciocalteu (F-C) reagent. The F-C assay relies on the transfer of electrons in alkaline medium from phenolic compounds to phosphomolybdic/phosphotungstic acid complexes, which are determined spectroscopically at 765 nm. Although the electron transfer reaction is not specific for phenolic compounds, the extraction procedure eliminates approximately 85% of ascorbic acid and other potentially interfering compounds. This assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. At least 64 samples can be processed in 1 d.
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