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  • Title: [Effects of L. lactis recombinant heme oxygenase-1 gene on the intestinal barrier in rats with hemorrhagic shock].
    Author: Gao XY, Ren CC, Zhang X, Yao YH, Pang QF, Wu CY.
    Journal: Zhongguo Wei Zhong Bing Ji Jiu Yi Xue; 2007 Apr; 19(4):225-8. PubMed ID: 17448278.
    Abstract:
    OBJECTIVE: To evaluate the effect of gavage of L. lactis recombinant heme oxygenase-1 (HO-1) gene on protection of the intestinal mucosa and the inflammation of lower intestine during hemorrhagic shock. METHODS: A model of hemorrhagic shock was reproduced in 30 SD healthy male rats. They were randomly divided into the L. lactis recombinant HO-1 gene group (HO group, n=10), L. lactis group (LL group, n=10), and glutamine group (Glu group, n=10). Glu was gavaged 6 hours and other agents were gavaged 24 hours before the experiment. Samples were collected 1 hour after hemorrhagic shock and fluid resuscitation. The mortality, mean arterial pressure (MAP) during hemorrhagic shock and fluid resuscitation, myeloperoxidase (MPO) activity, the pathological changes, and the contents of HO-1, tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10) of the lower intestine were determined immunohistochemically and compared. RESULTS: When the results of HO group were compared with Glu and LL groups, the mortality was significantly decreased in the former (both P<0.05). In HO group, MAP values at 5, 10, 20 and 30 minutes after fluid resuscitation were significantly elevated (all P<0.05). Compared with LL group, the gray levels of IL-10 and HO-1 in HO group and Glu group were significantly increased (all P<0.05). Compared with Glu group, the gray level of HO-1 was significantly increased in HO group (P<0.05). There were no significant differences in the gray levels of TNF-alpha among three groups. The Chiu's grade of HO group [(1.41+/-0.28) scores] was significantly lower than those of LL group and Glu group [(1.93+/-0.49) scores and (1.75+/-0.58) scores, respectively, both P<0.05]. CONCLUSION: The L. lactis recombinant HO-1 has the virtue to deliver HO-1 activity in rats with hemorrhagic shock, which is beneficial for the maintenance of intestinal barrier and anti-inflammation response of the lower intestine.
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