These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Establishment of urinary proteomic map on two-dimensional polyacrylamide gel electrophoresis in children with idiopathic nephrotic syndrome].
    Author: Ding JJ, He QN, Zhou P, Zhang L, Yi ZW.
    Journal: Zhongguo Dang Dai Er Ke Za Zhi; 2007 Apr; 9(2):122-4. PubMed ID: 17448306.
    Abstract:
    OBJECTIVE: To establish a urinary proteomic map on two-dimensional polyacrylamide gelelectrophoresis (2-DE) in children with idiopathic nephrotic syndrome (INS). METHODS: The proteins from INS children were purified by four various means, separated by 2-DEand stained by silver. RESULTS: The sequential preparation of urinary proteins by acetone precipitation and dislysis, when the sample was 300 microg, resulted in a clear background, well-resolved and reproducible 2-DE urinary protemic map in children with INS. CONCLUSIONS: A steady 2-DE technique for urinary protemic map in children with INS was established, which can be effectively applied in urinary proteomics of the disease.
    [Abstract] [Full Text] [Related] [New Search]