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  • Title: Cloning and characterization of a novel Delta12-fatty acid desaturase gene from the tree Sapium sebiferum.
    Author: Niu B, Ye H, Xu Y, Wang S, Chen P, Peng S, Ou Y, Tang L, Chen F.
    Journal: Biotechnol Lett; 2007 Jun; 29(6):959-64. PubMed ID: 17450326.
    Abstract:
    A new full-length cDNA (Ssd12) encoding a Delta12-fatty acid desaturase (Delta12-FAD) was cloned from Sapium sebiferum using RT-PCR and RACE methods. Ssd12 contained a 1146 bp open reading frame encoding a protein of 381 amino acids. The amino acid sequence showed a much higher match with microsomal Delta12-FAD amino acid sequences than chloroplast Delta12-FAD amino acid sequences. Genomic Southern blot analysis suggested that Ssd12 had at least two copies. Ssd12 transcripts were detected in roots, leaves, stems, and seeds by real time PCR.
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