These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Construction of the enhanced green fluorescent protein eukaryotic expression vector carrying CagA of Helicobacter pylori].
    Author: Feng BS, Yao XH, Zhao GQ.
    Journal: Zhonghua Yi Xue Za Zhi; 2007 Feb 27; 87(8):570-2. PubMed ID: 17459214.
    Abstract:
    OBJECTIVE: To construct a eukaryotic green fluorescent protein expressing vector containing the fragment of cytotoxin associated gene A (CagA) of Helicobacter pylori (Hp) so as to lay a foundation for the research of gene vaccine of gastric cancer. pEGFP-C3-CagA. METHODS: The fragment of gene CagA was amplified from Hp using PCR. The amplified product was examined by electrophoresis and sequence determination. This fragment was inserted into pGEM-T plasmid and pEGFP-C3 fluorescent expression vector. The recombined plasmid pEGFP-C3-CagA was transfected into the gastric carcinoma cells of the strain BGC823 by lipoplasty method. Fluorescence microscopy was used to observe the expression of pEGFP-C3-CagA under. RESULTS: CagA was inserted in the plasmid correctly. It was verified by DNA sequencing and restriction enzyme. The enhanced green fluorescent protein eukaryotic expression vector carrying CagA of Helicobacter pylori (pEGFP-C3-CagA) was recombined correctly and transfected in gastric carcinoma cell strain BGC823. Green fluorescence was observed in transfected gastric carcinoma cell. CONCLUSION: Construction of the enhanced green fluorescent protein eukaryotic expression vector carrying CagA was successful.
    [Abstract] [Full Text] [Related] [New Search]