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  • Title: Application and field validation of a PCR assay for the detection of Mycoplasma hyopneumoniae from swine lung tissue samples.
    Author: Cai HY, van Dreumel T, McEwen B, Hornby G, Bell-Rogers P, McRaild P, Josephson G, Maxie G.
    Journal: J Vet Diagn Invest; 2007 Jan; 19(1):91-5. PubMed ID: 17459839.
    Abstract:
    A PCR assay was validated for the detection of Mycoplasma hyopneumoniae in porcine lung tissue. The detection limit of the assay was 0.18 colony-forming units/g of lung sample spiked with M. hyopneumoniae. In field validation, 426 pigs from 220 cases were examined for M. hyopneumoniae infection by M. hyopneumoniae PCR and a fluorescent antibody (FA) test. In total, 103 pig lungs (24.2%) were positive in the PCR test, and 69 pig lungs (16.2%) were positive in the FA test, among which, 62 pigs were positive for both PCR and FA test. Most of the PCR-positive but FA test-negative cases had lesions compatible with M. hyopneumoniae infection. With Bayesian modeling, the diagnostic sensitivity and specificity of the PCR were determined to be 97.3% and 93.0%, respectively.
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